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ADGRB3 ELISA Kit (Mouse)

CAT: 0247-OKEH05638-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH05638-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Adhesion G protein-coupled receptor B3
Gene Aliases
A830096D10Rik; adhesion G protein-coupled receptor B3; Ba; Bai3; brain-specific angiogenesis inhibitor 3; G protein-coupled receptor BAI3.
Gene ID
210933
Accession Number
NP_783573.4
Reactivity
Mouse, Mus musculus
Target
Adhesion G protein-coupled receptor B3
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Adgrb3 ELISA Kit (Mouse) (OKEH05638) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Adgrb3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Adgrb3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Adgrb3 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 109.8%
Sensitivity
0.161 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Brain-specific angiogenesis inhibitor 3
Protein Length
1522
NCBI Gene Symbol
Adgrb3
Protein Name
Q80ZF8
Gene Name URL
Adgrb3
Nucleotide Accession Number
NM_175642.4

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