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KRT20 ELISA Kit (Mouse)

CAT: 0247-OKEH05600-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH05600-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Keratin 20
Gene Aliases
9030623C06Rik; Ck20; Ck-20; cytokeratin 20; Cytokeratin-20; K20; keratin, type I cytoskeletal 20; Keratin-20; Krt21.
Gene ID
66809
Accession Number
NP_075745.1
Reactivity
Mouse, Mus musculus
Target
Keratin, type I cytoskeletal 20
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Krt20 ELISA Kit (Mouse) (OKEH05600) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Krt20 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Krt20 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Krt20 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 83.9%
Sensitivity
39.5 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Keratin, type I cytoskeletal 20
Protein Length
431
NCBI Gene Symbol
Krt20
Protein Name
Q9D312
Gene Name URL
Krt20
Nucleotide Accession Number
NM_023256.2

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