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ATP5B ELISA Kit (Mouse)

CAT: 0247-OKEH05591-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH05591-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
ATP synthase, H+ transporting mitochondrial F1 complex, beta subunit
Gene Aliases
ATP synthase F1 subunit beta; ATP synthase subunit beta, mitochondrial; ATP synthase, H+ transporting mitochondrial F1 complex, alpha subunit; Atp5f1b; mitochondrial ATP synthase, H+ transporting F1 complex beta subunit.
Gene ID
11947
Accession Number
NP_058054.2
Reactivity
Mouse, Mus musculus
Target
ATP synthase subunit beta, mitochondrial
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Atp5b ELISA Kit (Mouse) (OKEH05591) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Atp5b has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Atp5b is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Atp5b captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
31.2-2000pg/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 85.9%
Sensitivity
15.6 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse ATP synthase subunit beta, mitochondrial
Protein Length
529
NCBI Gene Symbol
Atp5b
Protein Name
P56480
Gene Name URL
Atp5b
Nucleotide Accession Number
NM_016774.3

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