CD46 ELISA Kit (Mouse)

CAT: 0247-OKEH05581-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH05581-96WSize:96 Well
Selected
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product image 1
1 / 1
Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
CD46 antigen, complement regulatory protein
Gene Aliases
Mc; Mcp; membrane cofactor protein.
Gene ID
17221
Accession Number
NP_034908.1
Reactivity
Mouse, Mus musculus
Target
Membrane cofactor protein
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Cd46 ELISA Kit (Mouse) (OKEH05581) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cd46 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Cd46 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cd46 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
15.6-1000pg/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 93%
Sensitivity
8.08 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Membrane cofactor protein
Protein Length
365
NCBI Gene Symbol
Cd46
Protein Name
O88174
Gene Name URL
Cd46
Nucleotide Accession Number
NM_010778.4

Popular Products