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EPCAM ELISA Kit (Mouse)

CAT: 0247-OKEH05578-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH05578-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
_x005F Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Epithelial cell adhesion molecule
Gene Aliases
CD326; EGP; EGP-; EGP-2; Egp31; Egp314; EpC; Ep-C; EpCA; Ep-CAM; EpCAM1; epithelial cell adhesion molecule; epithelial glycoprotein 314; GA733-; GA733-2; gp4; gp40; Ly7; Ly74; lymphocyte antigen 74; mEGP314; panepithelial glycoprotein 314; protein 289A; Tacsd1; Tacst; Tacstd1; TR; TROP1; Trop-1 protein; tumor-associated calcium signal transducer 1.
Gene ID
17075
Accession Number
NP_032558.2
Reactivity
Mouse, Mus musculus
Target
Epithelial cell adhesion molecule
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Epcam ELISA Kit (Mouse) (OKEH05578) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Epcam has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Epcam is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Epcam captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 90%
Sensitivity
0.176 ng/mL
Reconstitution
Store as indicated in product manual.
Notes
Formerly GWB-QTMESF
Specificity
Natural and recombinant Mouse Epithelial cell adhesion molecule
Protein Length
315
NCBI Gene Symbol
Epcam
Protein Name
Q99JW5
Gene Name URL
Epcam
Nucleotide Accession Number
NM_008532.2

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