PRPSAP1 ELISA Kit (Mouse)

CAT: 0247-OKEH05564-96WSize: 96 TestsDry Ice: NoHazardous: No
CAT#:0247-OKEH05564-96WSize:96 Tests
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Phosphoribosyl pyrophosphate synthetase-associated protein 1
Gene Aliases
PA, PAP39, 5730409F23Rik
Gene ID
67763
Accession Number
NP_080640.1
Reactivity
Mouse
Target
Seems to play a negative regulatory role in 5-phosphoribose 1-diphosphate synthesis.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Prpsap1 ELISA Kit (Mouse) (OKEH05564) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Prpsap1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Prpsap1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Prpsap1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
78 - 5000pg/ml
Recovery
Mean recovery when spiking into Serum and Plasma = 112.7%
Sensitivity
39.9 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Phosphoribosyl pyrophosphate synthase-associated protein 1
Protein Length
356
NCBI Gene Symbol
PRPSAP1
Protein Name
Phosphoribosyl pyrophosphate synthase-associated protein 1
Gene Name URL
PRPSAP1
Nucleotide Accession Number
NM_026364.1

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