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MTHFD1L ELISA Kit (Human)

CAT: 0247-OKEH05556-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH05556-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like
Gene Aliases
10-formyl-THF synthetase; dJ292B18.2; Formyltetrahydrofolate synthetase; formyltetrahydrofolate synthetase domain containing 1; FTHFSDC1; monofunctional C1-tetrahydrofolate synthase, mitochondrial; MTC1THFS.
Gene ID
25902
Accession Number
NP_001229696.1
Reactivity
Homo sapiens, Human
Target
Monofunctional C1-tetrahydrofolate synthase, mitochondrial
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology MTHFD1L ELISA Kit (Human) (OKEH05556) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MTHFD1L has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MTHFD1L is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MTHFD1L captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
1.56-100ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 111%
Sensitivity
0.12 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Monofunctional C1-tetrahydrofolate synthase, mitochondrial
Protein Length
978
NCBI Gene Symbol
MTHFD1L
Protein Name
Q6UB35
Gene Name URL
MTHFD1L
Nucleotide Accession Number
NM_001242767.1

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