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ATF1 ELISA Kit (Mouse)

CAT: 0247-OKEH05550-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH05550-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Activating transcription factor 1
Gene Aliases
Activating transcription factor 1; cAMP-dependent transcription factor ATF-1; cyclic AMP-dependent transcription factor ATF-1; TCR-ATF1.
Gene ID
11908
Accession Number
NP_031523.3
Reactivity
Mouse, Mus musculus
Target
Cyclic AMP-dependent transcription factor ATF-1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Atf1 ELISA Kit (Mouse) (OKEH05550) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Atf1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Atf1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Atf1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
15.6-1000pg/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 105.5%
Sensitivity
7.86 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Cyclic AMP-dependent transcription factor ATF-1
Protein Length
269
NCBI Gene Symbol
Atf1
Protein Name
P81269
Gene Name URL
Atf1
Nucleotide Accession Number
NM_007497.3

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