CIB1 ELISA Kit (Mouse)

• Reproducibility:

  Mean Inter-Assay Precision -

• Gene Name:

  Calcium and integrin binding 1 (calmyrin)

• Gene Aliases:

  Calcium and integrin binding, protein kinase interacting protein; calcium and integrin-binding protein 1; calmyrin; CIB; Cibkip; DNA-PKcs-interacting protein; K; kinase-interacting protein; Kip; Prkdcip; protein kinase, DNA activated, catalytic polypeptide interacting protein.

• Gene ID:

  23991

• Accession Number:

  NP_001278204.1

• Reactivity:

  Mouse, Mus musculus

• Target:

  Calcium and integrin-binding protein 1

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Assay Principle:

  Aviva Systems Biology Cib1 ELISA Kit (Mouse) (OKEH05524) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cib1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Cib1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cib1 captured in the well.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, plasma and other biological fluids

• Detection Range:

  0.312-20ng/mL

• Recovery:

  Mean recovery when spiking into Serum and Plasma = 105%

• Sensitivity:

  0.176 ng/mL

• Reconstitution:

  Store as indicated in product manual.

• Specificity:

  Natural and recombinant Mouse Calcium and integrin-binding protein 1

• Protein Length:

  191

• NCBI Gene Symbol:

  Cib1

• Protein Name:

  Q9Z0F4

• Gene Name URL:

  Cib1

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_001291275.1