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NME1 ELISA Kit (Mouse)

CAT: 0247-OKEH05499-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH05499-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
NME/NM23 nucleoside diphosphate kinase 1
Gene Aliases
AL024257; expressed in non-metastatic cells 1, protein; metastasis inhibition factor NM23; NDK A; NDP; NDP kinase A; NDPK-A; NM23A; NM23-M; NM23-M1; non-metastatic cells 1, protein (NM23A) expressed in; nucleoside diphosphate kinase A; nucleoside-diphosphate kinase 1; nucleotide diphosphate kinase; tumor metastatic process-associated protein.
Gene ID
18102
Accession Number
NP_032730.1
Reactivity
Mouse, Mus musculus
Target
Nucleoside diphosphate kinase A
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Nme1 ELISA Kit (Mouse) (OKEH05499) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Nme1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Nme1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Nme1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 93.7%
Sensitivity
0.089 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Nucleoside diphosphate kinase A
Protein Length
152
NCBI Gene Symbol
Nme1
Protein Name
P15532
Gene Name URL
Nme1
Nucleotide Accession Number
NM_008704.2

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