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PIK3CD ELISA Kit (Mouse)

CAT: 0247-OKEH05498-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH05498-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta
Gene Aliases
2410099E07Rik;2610208K16Rik; AW545373; catalytic phosphatidylinositol 3-kinase delta; p100 delta; p110de; p110delta; phosphatidylinositol 3-kinase catalytic delta polypeptide; phosphatidylinositol 3-kinase catalytic subunit delta; phosphatidylinositol 3-kinase p110 delta; phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit delta; phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform; phosphoinositide 3 kinase P110delta; PI3Kdelta; PI3K-delta; PI3-kinase subunit delta; ptdIns-3-kinase subunit delta; ptdIns-3-kinase subunit p110-delta.
Gene ID
18707
Accession Number
NP_001157524.1
Reactivity
Mouse, Mus musculus
Target
Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Pik3cd ELISA Kit (Mouse) (OKEH05498) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pik3cd has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Pik3cd is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pik3cd captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 90%
Sensitivity
0.184 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform
Protein Length
1043
NCBI Gene Symbol
Pik3cd
Protein Name
O35904
Gene Name URL
Pik3cd
Nucleotide Accession Number
NM_001164052.1

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