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NLRP3 ELISA Kit (Mouse)

CAT: 0247-OKEH05486-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH05486-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
_x005F Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
NLR family, pyrin domain containing 3
Gene Aliases
AGTAVPRL; AII/AVP; Ci; Cias1; cold autoinflammatory syndrome 1 protein homolog; cry; cryopyrin; FCAS; FCU; mast cell maturation-associated-inducible protein 1; Mmig; Mmig1; MWS; N; NACHT, LRR and PYD domains-containing protein 3; NACHT/LRR/pyrin domain-containing protein 3; NALP3; Pyp; Pypaf1; PYRIN-containing APAF1-like protein 1.
Gene ID
216799
Accession Number
NP_665826.1
Reactivity
Mouse, Mus musculus
Target
NACHT, LRR and PYD domains-containing protein 3
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Nlrp3 ELISA Kit (Mouse) (OKEH05486) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Nlrp3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Nlrp3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Nlrp3 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 94%
Sensitivity
0.058 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse NACHT, LRR and PYD domains-containing protein 3
Protein Length
1033
NCBI Gene Symbol
Nlrp3
Protein Name
Q8R4B8
Gene Name URL
Nlrp3
Nucleotide Accession Number
NM_145827.3