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MTOR ELISA Kit (Mouse)

CAT: 0247-OKEH05454-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH05454-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Mechanistic target of rapamycin kinase
Gene Aliases
2610315D21Rik; AI327068; angiopoietin-like factor CDT6; FK506 binding protein 12-rapamycin associated protein 1; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; FKBP-rapamycin associated protein (FRAP) ; fl; flat; Fr; FRAP; Frap1; FRAP2; mammalian target of rapamycin; Mechanistic target of rapamycin; mechanistic target of rapamycin (serine/threonine kinase) ; RA; RAF; RAFT1; rapamycin target protein 1; RAPT1; serine/threonine-protein kinase mTOR.
Gene ID
56717
Accession Number
NP_064393.2
Reactivity
Mouse, Mus musculus
Target
Serine/threonine-protein kinase mTOR
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Mtor ELISA Kit (Mouse) (OKEH05454) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Mtor has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Mtor is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Mtor captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 90.8%
Sensitivity
39.3 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Serine/threonine-protein kinase mTOR
Protein Length
2549
NCBI Gene Symbol
Mtor
Protein Name
Q9JLN9
Gene Name URL
Mtor
Nucleotide Accession Number
NM_020009.2

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