MTOR ELISA Kit (Mouse)

• Reproducibility:

  Mean Inter-Assay Precision -

• Gene Name:

  Mechanistic target of rapamycin kinase

• Gene Aliases:

  2610315D21Rik; AI327068; angiopoietin-like factor CDT6; FK506 binding protein 12-rapamycin associated protein 1; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; FKBP-rapamycin associated protein (FRAP) ; fl; flat; Fr; FRAP; Frap1; FRAP2; mammalian target of rapamycin; Mechanistic target of rapamycin; mechanistic target of rapamycin (serine/threonine kinase) ; RA; RAF; RAFT1; rapamycin target protein 1; RAPT1; serine/threonine-protein kinase mTOR.

• Gene ID:

  56717

• Accession Number:

  NP_064393.2

• Reactivity:

  Mouse, Mus musculus

• Target:

  Serine/threonine-protein kinase mTOR

• Type:

  ELISA Kit

• Applications:

  Enzyme-linked Immunosorbent assay-Sandwich

• Assay Principle:

  Aviva Systems Biology Mtor ELISA Kit (Mouse) (OKEH05454) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Mtor has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Mtor is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Mtor captured in the well.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, plasma and other biological fluids

• Detection Range:

  78-5000pg/mL

• Recovery:

  Mean recovery when spiking into Serum and Plasma = 90.8%

• Sensitivity:

  39.3 pg/mL

• Reconstitution:

  Store as indicated in product manual.

• Specificity:

  Natural and recombinant Mouse Serine/threonine-protein kinase mTOR

• Protein Length:

  2549

• NCBI Gene Symbol:

  Mtor

• Protein Name:

  Q9JLN9

• Gene Name URL:

  Mtor

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_020009.2