NT5C3B ELISA Kit (Mouse)

• Reproducibility:

  Mean Inter-Assay Precision -

• Gene Name:

  5'-nucleotidase, cytosolic IIIB

• Gene Aliases:

  Nt5c, Nt5c3l, AI841843, 2610037D24Rik, C330027I04Rik

• Gene ID:

  68106

• Accession Number:

  NP_001096120.1

• Reactivity:

  Mouse

• Target:

  Specifically hydrolyzes 7-methylguanosine monophosphate (m7GMP) to 7-methylguanosine and inorganic phosphate. The specific activity for m7GMP may protect cells against undesired salvage of m7GMP and its incorporation into nucleic acids. Also has weak activity for CMP. UMP and purine nucleotides are poor substrates.

• Type:

  ELISA Kit

• Assay Principle:

  Aviva Systems Biology Nt5c3b ELISA Kit (Mouse) (OKEH05452) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Nt5c3b has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Nt5c3b is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Nt5c3b captured in the well.

• Assay Protocol:

  Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

• Sample Type:

  Serum, plasma and other biological fluids

• Detection Range:

  0.312 - 20ng/ml

• Recovery:

  Mean recovery when spiking into Serum and Plasma = 93.1%

• Sensitivity:

  0.18 ng/mL

• Reconstitution:

  Store as indicated in product manual.

• Specificity:

  Natural and recombinant Mouse Cytosolic 5'-nucleotidase III-like protein

• Protein Length:

  300

• NCBI Gene Symbol:

  NT5C3B

• Protein Name:

  7-methylguanosine phosphate-specific 5'-nucleotidase

• Gene Name URL:

  NT5C3B

• CAS Number:

  7732-18-5

• Nucleotide Accession Number:

  NM_001102650.1