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TCL1A ELISA Kit (Human)

CAT: 0247-OKEH05366-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH05366-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
TCL1 family AKT coactivator A
Gene Aliases
Oncogene TCL1; oncogene TCL-1; protein p14 TCL1; T cell leukemia/lymphoma 1A; T-cell leukemia/lymphoma protein 1A; T-cell lymphoma-1; TCL1.
Gene ID
8115
Accession Number
NP_001092195.1
Reactivity
Homo sapiens, Human
Target
T-cell leukemia/lymphoma protein 1A
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology TCL1A ELISA Kit (Human) (OKEH05366) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TCL1A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TCL1A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TCL1A captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 96%
Sensitivity
39.3 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human T-cell leukemia/lymphoma protein 1A
Protein Length
114
NCBI Gene Symbol
TCL1A
Protein Name
P56279
Gene Name URL
TCL1A
Nucleotide Accession Number
NM_001098725.1

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