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MAP2K6 ELISA Kit (Human)

CAT: 0247-OKEH05362-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH05362-96WSize:96 Well
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Mitogen-activated protein kinase kinase 6
Gene Aliases
Dual specificity mitogen-activated protein kinase kinase 6; MAPK/ERK kinase 6; MAPKK 6; MAPKK6; MEK 6; MEK6; MKK6; PRKMK6; protein kinase, mitogen-activated, kinase 6 (MAP kinase kinase 6) ; SAPK kinase 3; SAPKK3; SAPKK-3; stress-activated protein kinase kinase 3.
Gene ID
5608
Accession Number
NP_002749.2
Reactivity
Homo sapiens, Human
Target
Dual specificity mitogen-activated protein kinase kinase 6
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology MAP2K6 ELISA Kit (Human) (OKEH05362) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MAP2K6 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MAP2K6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MAP2K6 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 103%
Sensitivity
0.092 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Dual specificity mitogen-activated protein kinase kinase 6
Protein Length
334
NCBI Gene Symbol
MAP2K6
Protein Name
P52564
Gene Name URL
MAP2K6
Nucleotide Accession Number
NM_002758.3

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