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PLSCR1 ELISA Kit (Mouse)

CAT: 0247-OKEH05334-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH05334-96WSize:96 Well
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Phospholipid scramblase 1
Gene Aliases
Ca (2+) -dependent phospholipid scramblase 1; mg (2+) -dependent nuclease; MmTR; MmTRA; MmTRA1a; MmTRA1b; MuPL; N; Nor1; phospholipid scramblase 1; PL scramblase 1; TR; Tra1; Tra1a; Tra1b; transplantability-associated protein 1; Tras1; Tras2.
Gene ID
22038
Accession Number
NP_035766.2
Reactivity
Mouse, Mus musculus
Target
Phospholipid scramblase 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Plscr1 ELISA Kit (Mouse) (OKEH05334) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Plscr1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Plscr1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Plscr1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.625-40ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 86.2%
Sensitivity
0.332 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Phospholipid scramblase 1
Protein Length
328
NCBI Gene Symbol
Plscr1
Protein Name
Q9JJ00
Gene Name URL
Plscr1
Nucleotide Accession Number
NM_011636.2

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