RIPK2 ELISA Kit (Mouse)

CAT: 0247-OKEH05329-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH05329-96WSize:96 Well
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Receptor (TNFRSF) -interacting serine-threonine kinase 2
Gene Aliases
2210420D18Rik; CAR; CARD3; CARDIAK; CCK; D4Bwg0615e; R; receptor-interacting serine/threonine-protein kinase 2; RI; RICK; RIP2; tyrosine-protein kinase RIPK2.
Gene ID
192656
Accession Number
NP_620402.1
Reactivity
Mouse, Mus musculus
Target
Receptor-interacting serine/threonine-protein kinase 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Ripk2 ELISA Kit (Mouse) (OKEH05329) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ripk2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ripk2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ripk2 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 94.1%
Sensitivity
0.082 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Receptor-interacting serine/threonine-protein kinase 2
Protein Length
539
NCBI Gene Symbol
Ripk2
Protein Name
P58801
Gene Name URL
Ripk2
Nucleotide Accession Number
NM_138952.4

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