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SPA17 ELISA Kit (Human)

CAT: 0247-OKEH05288-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH05288-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Sperm autoantigenic protein 17
Gene Aliases
Cancer/testis antigen 22; CT22; SP17; SP17-1; Sperm autoantigenic protein 17; sperm protein 17; sperm surface protein Sp17.
Gene ID
53340
Accession Number
NP_059121.1
Reactivity
Homo sapiens, Human
Target
Sperm surface protein Sp17
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology SPA17 ELISA Kit (Human) (OKEH05288) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SPA17 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SPA17 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SPA17 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 99%
Sensitivity
40.6 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Sperm surface protein Sp17
Protein Length
151
NCBI Gene Symbol
SPA17
Protein Name
Q15506
Gene Name URL
SPA17
Nucleotide Accession Number
NM_017425.3

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