C8A ELISA Kit (Mouse)

CAT: 0247-OKEH05281-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH05281-96WSize:96 Well
Selected
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product image 1
1 / 1
Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Complement component 8, alpha polypeptide
Gene Aliases
Complement component 8 alpha subunit; Complement component 8 subunit alpha; complement component C8 alpha chain.
Gene ID
230558
Accession Number
NP_666260.1
Reactivity
Mouse, Mus musculus
Target
Complement component C8 alpha chain
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology C8a ELISA Kit (Mouse) (OKEH05281) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for C8a has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for C8a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample C8a captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
1.56-100ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 97.8%
Sensitivity
0.78 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Complement component C8 alpha chain
Protein Length
587
NCBI Gene Symbol
C8a
Protein Name
Q8K182
Gene Name URL
C8a
Nucleotide Accession Number
NM_146148.2

Popular Products