Products for Research Use Only

EDNRA ELISA Kit (Mouse)

CAT: 0247-OKEH05275-96WSize: 96 WellDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0247-OKEH05275-96WSize:96 Well
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Endothelin receptor type A
Gene Aliases
AEA0; AEA001; endothelin A receptor; Endothelin receptor type A; endothelin-1 receptor; ET; ET-; ETa; ET-A; ET-AR; Gpcr; Gpcr10; G-protein coupled receptor 10; Mhdaa; Mhdaaea1.
Gene ID
13617
Accession Number
NP_034462.1
Reactivity
Mouse, Mus musculus
Target
Endothelin-1 receptor
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Ednra ELISA Kit (Mouse) (OKEH05275) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ednra has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ednra is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ednra captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 100.8%
Sensitivity
0.094 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Endothelin-1 receptor
Protein Length
427
NCBI Gene Symbol
Ednra
Protein Name
Q61614
Gene Name URL
Ednra
Nucleotide Accession Number
NM_010332.2