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ADAMTS2 ELISA Kit (Human)

CAT: 0247-OKEH05252-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH05252-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
ADAM metallopeptidase with thrombospondin type 1 motif 2
Gene Aliases
A disintegrin and metalloproteinase with thrombospondin motifs 2; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 2; ADAMTS-2; ADAM-TS2; ADAMTS-3; EDSDERMS; NPI; PC I-NP; PCINP; PCI-NP; PCPNI; PNPI; procollagen I N-proteinase; procollagen I/II amino propeptide-processing enzyme; procollagen N-endopeptidase.
Gene ID
9509
Accession Number
NP_055059.2
Reactivity
Homo sapiens, Human
Target
A disintegrin and metalloproteinase with thrombospondin motifs 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology ADAMTS2 ELISA Kit (Human) (OKEH05252) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ADAMTS2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ADAMTS2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ADAMTS2 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 100%
Sensitivity
39 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human A disintegrin and metalloproteinase with thrombospondin motifs 2
Protein Length
1211
NCBI Gene Symbol
ADAMTS2
Protein Name
O95450
Gene Name URL
ADAMTS2
Nucleotide Accession Number
NM_014244.4

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