STOM ELISA Kit (Mouse)
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STOM ELISA Kit (Mouse)
Reproducibility:
_x005F Mean Inter-Assay Precision -Gene Name:
StomatinGene Aliases:
Epb7.; Epb7.2; erythrocyte band 7 integral membrane protein; erythrocyte membrane protein band 7.2; erythrocyte protein band 7.2; erythrocyte protein band 7.2; protein 7.2b; protein 7.2b; sto; stomatin.Gene ID:
13830Accession Number:
NP_038543.1Reactivity:
Mouse, Mus musculusTarget:
Erythrocyte band 7 integral membrane proteinType:
ELISA KitApplications:
Enzyme-linked Immunosorbent assay-SandwichAssay Principle:
Aviva Systems Biology Stom ELISA Kit (Mouse) (OKEH05242) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Stom has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Stom is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Stom captured in the well.Assay Protocol:
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) ProtocolSample Type:
Serum, plasma and other biological fluidsDetection Range:
0.312-20ng/mLSensitivity:
0.189 ng/mLReconstitution:
Store as indicated in product manual.Specificity:
Natural and recombinant Mouse Erythrocyte band 7 integral membrane proteinProtein Length:
284NCBI Gene Symbol:
StomProtein Name:
P54116Gene Name URL:
StomCAS Number:
7732-18-5Nucleotide Accession Number:
NM_013515.2
