STOM ELISA Kit (Mouse)

CAT: 0247-OKEH05242-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH05242-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
_x005F Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Stomatin
Gene Aliases
Epb7.; Epb7.2; erythrocyte band 7 integral membrane protein; erythrocyte membrane protein band 7.2; erythrocyte protein band 7.2; erythrocyte protein band 7.2; protein 7.2b; protein 7.2b; sto; stomatin.
Gene ID
13830
Accession Number
NP_038543.1
Reactivity
Mouse, Mus musculus
Target
Erythrocyte band 7 integral membrane protein
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Stom ELISA Kit (Mouse) (OKEH05242) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Stom has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Stom is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Stom captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.312-20ng/mL
Sensitivity
0.189 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Erythrocyte band 7 integral membrane protein
Protein Length
284
NCBI Gene Symbol
Stom
Protein Name
P54116
Gene Name URL
Stom
Nucleotide Accession Number
NM_013515.2