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CSF3R ELISA Kit (Mouse)

CAT: 0247-OKEH05213-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH05213-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Colony stimulating factor 3 receptor (granulocyte)
Gene Aliases
Cd114; Csf; Csfgr; G-CS; G-CSF receptor; G-CSFR; G-CSF-R; granulocyte colony-stimulating factor receptor.
Gene ID
12986
Accession Number
NP_031808.2
Reactivity
Mouse, Mus musculus
Target
Granulocyte colony-stimulating factor receptor
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Csf3r ELISA Kit (Mouse) (OKEH05213) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Csf3r has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Csf3r is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Csf3r captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
31.2-2000pg/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 105.6%
Sensitivity
15.79 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Granulocyte colony-stimulating factor receptor
Protein Length
837
NCBI Gene Symbol
Csf3r
Protein Name
P40223
Gene Name URL
Csf3r
Nucleotide Accession Number
NM_007782.3

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