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NDST2 ELISA Kit (Mouse)

CAT: 0247-OKEH05208-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH05208-96WSize:96 Well
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 2
Gene Aliases
[Heparan sulfate]-glucosamine N-sulfotransferase; bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 2; glucosaminyl N-deacetylase/N-sulfotransferase 2; glucosaminyl N-deacetylase/N-sulphotransferase 2; heparan glucosaminyl N-deacetylase/N-sulfotransferase 2; heparan N-sulfotransferase 2; heparan sulfate N-deacetylase/N-sulfotransferase 2; mastocytoma N-deacetylase/N-sulfotransferase; Mn; Mndns; NDST-2; N-heparan sulfate sulfotransferase 2; N-HSST 2.
Gene ID
17423
Accession Number
NP_034941.2
Reactivity
Mouse, Mus musculus
Target
Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Ndst2 ELISA Kit (Mouse) (OKEH05208) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ndst2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ndst2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ndst2 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 97.8%
Sensitivity
42.5 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 2
Protein Length
883
NCBI Gene Symbol
Ndst2
Protein Name
P52850
Gene Name URL
Ndst2
Nucleotide Accession Number
NM_010811.2

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