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PMEL ELISA Kit (Mouse)

CAT: 0247-OKEH05173-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH05173-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Melanocyte protein PMEL
Gene Aliases
Si, gp8, Silv, gp10, gp87, gp100, Pmel17, D12S53E, D12S53Eh, D10H12S53, D10H12S53E
Gene ID
20431
Accession Number
NP_068682.2
Reactivity
Mouse
Target
Plays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Pmel ELISA Kit (Mouse) (OKEH05173) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pmel has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Pmel is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pmel captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
78 - 5000pg/ml
Recovery
Mean recovery when spiking into Serum and Plasma = 105%
Sensitivity
39.3 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Melanocyte protein PMEL
Protein Length
626
NCBI Gene Symbol
PMEL
Protein Name
Melanocyte protein PMEL
Gene Name URL
PMEL
Nucleotide Accession Number
NM_021882.4

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