MAP1LC3A ELISA Kit (Mouse)

CAT: 0247-OKEH05147-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH05147-96WSize:96 Well
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Microtubule-associated protein 1 light chain 3 alpha
Gene Aliases
1010001H21Rik;4922501H04Rik; autophagy-related protein LC3 A; autophagy-related ubiquitin-like modifier LC3 A; LC; LC3; LC3a; MAP1 light chain 3-like protein 1; MAP1A/MAP1B LC3 A; MAP1A/MAP1B light chain 3 A; Microtubule-associated protein 1 light chain 3 alpha; microtubule-associated proteins 1A/1B light chain 3A.
Gene ID
66734
Accession Number
NP_080011.1
Reactivity
Mouse, Mus musculus
Target
Microtubule-associated proteins 1A/1B light chain 3A
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Map1lc3a ELISA Kit (Mouse) (OKEH05147) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Map1lc3a has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Map1lc3a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Map1lc3a captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.78-50ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 89.6%
Sensitivity
0.41 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Microtubule-associated proteins 1A/1B light chain 3A
Protein Length
121
NCBI Gene Symbol
Map1lc3a
Protein Name
Q91VR7
Gene Name URL
Map1lc3a
Nucleotide Accession Number
NM_025735.3

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