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TBC1D1 ELISA Kit (Mouse)

CAT: 0247-OKEH05121-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH05121-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
TBC1 domain family, member 1
Gene Aliases
1110062G02Rik; AI385682; AW555803; mKIAA1108; No; Nob; Nob1; Nobq1; Tbc1; TBC1 domain family member 1; tre-2/USP6, BUB2, cdc16) domain family, member 1.
Gene ID
57915
Accession Number
NP_001297540.1
Reactivity
Mouse, Mus musculus
Target
TBC1 domain family member 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Tbc1d1 ELISA Kit (Mouse) (OKEH05121) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Tbc1d1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Tbc1d1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Tbc1d1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 115.2%
Sensitivity
39.7 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse TBC1 domain family member 1
Protein Length
1255
NCBI Gene Symbol
Tbc1d1
Protein Name
Q60949
Gene Name URL
Tbc1d1
Nucleotide Accession Number
NM_001310611.1

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