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TBPL1 ELISA Kit (Mouse)

CAT: 0247-OKEH05120-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH05120-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
TATA box binding protein-like 1
Gene Aliases
21 kDa TBP-like protein;4732475G08; AW011832; AW491032; D18347; STD; T; TATA box binding protein-like protein; TATA box-binding protein-like 1; TATA box-binding protein-like protein 1; TATA box-binding protein-related factor 2; TBP-like 1; TBP-like factor; TBP-like protein 1; TBP-related factor 2; TBP-related protein; TL; TLF; Tlp; TRF; TRF2; TRP.
Gene ID
237336
Accession Number
NP_035733.1
Reactivity
Mouse, Mus musculus
Target
TATA box-binding protein-like protein 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Tbpl1 ELISA Kit (Mouse) (OKEH05120) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Tbpl1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Tbpl1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Tbpl1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 100.4%
Sensitivity
39.8 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse TATA box-binding protein-like protein 1
Protein Length
186
NCBI Gene Symbol
Tbpl1
Protein Name
P62340
Gene Name URL
Tbpl1
Nucleotide Accession Number
NM_011603.5

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