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NRGN ELISA Kit (Mouse)

CAT: 0247-OKEH05025-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH05025-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
_x005F Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Neurogranin
Gene Aliases
0710001B06Rik; AI838505; N; neurogranin; neurogranin (protein kinase C substrate, RC3) ; NG; NG/R; NG/RC3; P; postsynaptic spine 1; protein kinase C substrate; Pss1; R75334; RC; RC3.
Gene ID
64011
Accession Number
NP_071312.1
Reactivity
Mouse, Mus musculus
Target
Neurogranin
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Nrgn ELISA Kit (Mouse) (OKEH05025) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Nrgn has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Nrgn is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Nrgn captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 100%
Sensitivity
39.5 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Neurogranin
Protein Length
78
NCBI Gene Symbol
Nrgn
Protein Name
P60761
Gene Name URL
Nrgn
Nucleotide Accession Number
NM_022029.2

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