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IL2RG ELISA Kit (Mouse)

CAT: 0247-OKEH04993-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH04993-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Interleukin 2 receptor, gamma chain
Gene Aliases
[g]c; CD132; common gamma chain; cytokine receptor common subunit gamma; gamm; gamma C receptor; gamma (; gamma (c) ; gammaC; gc; IL-2 receptor subunit gamma; IL-2R subunit gamma; interleukin-2 receptor subunit gamma; p64.
Gene ID
16186
Accession Number
NP_038591.1
Reactivity
Mouse, Mus musculus
Target
Cytokine receptor common subunit gamma
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Il2rg ELISA Kit (Mouse) (OKEH04993) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Il2rg has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Il2rg is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Il2rg captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 83.9%
Sensitivity
41.3 pg/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Cytokine receptor common subunit gamma
Protein Length
369
NCBI Gene Symbol
Il2rg
Protein Name
P34902
Gene Name URL
Il2rg
Nucleotide Accession Number
NM_013563.4

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