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PTPRF ELISA Kit (Mouse)

CAT: 0247-OKEH04975-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH04975-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Protein tyrosine phosphatase, receptor type, F
Gene Aliases
AA591035; LAR; LARS; leukocyte antigen-related (LAR) PTP receptor; leukocyte common antigen related; receptor-type tyrosine-protein phosphatase F; RP; RPTP-LAR.
Gene ID
19268
Accession Number
NP_035343.2
Reactivity
Mouse, Mus musculus
Target
Receptor-type tyrosine-protein phosphatase F
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Ptprf ELISA Kit (Mouse) (OKEH04975) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ptprf has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ptprf is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ptprf captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
1.56-100ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 88.5%
Sensitivity
0.782 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Receptor-type tyrosine-protein phosphatase F
Protein Length
1898
NCBI Gene Symbol
Ptprf
Protein Name
A2A8L5
Gene Name URL
Ptprf
Nucleotide Accession Number
NM_011213.2

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