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DGKA ELISA Kit (Mouse)

CAT: 0247-OKEH04965-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH04965-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Reproducibility
Mean Inter-Assay Precision -
CAS Number
7732-18-5
Gene Name
Diacylglycerol kinase, alpha
Gene Aliases
80 kDa diacylglycerol kinase;80kDa; AW146112; DAG kinase alpha; Dagk; Dagk1; DGK-alpha; diacylglycerol kinase alpha; diacylglycerol kinase alpha Flt2-Pr2; diglyceride kinase alpha; truncated diacylglycerol kinase alpha Flt1-Pr1; truncated diacylglycerol kinase alpha Flt1-Pr2.
Gene ID
13139
Accession Number
NP_058091.2
Reactivity
Mouse, Mus musculus
Target
Diacylglycerol kinase alpha
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Dgka ELISA Kit (Mouse) (OKEH04965) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Dgka has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Dgka is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Dgka captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.625-40ng/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 81.3%
Sensitivity
0.316 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Mouse Diacylglycerol kinase alpha
Protein Length
730
NCBI Gene Symbol
Dgka
Protein Name
O88673
Gene Name URL
Dgka
Nucleotide Accession Number
NM_016811.2

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