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CRYAA ELISA Kit (Human)

CAT: 0247-OKEH04871-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04871-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Crystallin alpha A, crystallin alpha A2
Gene Aliases
Alpha-crystallin A chain; alpha-crystallin A2 chain; CRYA1; CRYAA; CRYAA2; crystallin, alpha-1; CTRCT9; heat shock protein beta-4; HSPB4; human alphaA-crystallin (CRYA1) .
Gene ID
102724652, 1409
Accession Number
NP_000385.1
Reactivity
Homo sapiens, Human
Target
Alpha-crystallin A chain
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CRYAA ELISA Kit (Human) (OKEH04871) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CRYAA has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CRYAA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CRYAA captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
0.78-50ng/mL
Sensitivity
0.23 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
20 kDa
Specificity
Natural and recombinant Human Alpha-crystallin A chain
Protein Length
173
NCBI Gene Symbol
CRYAA, LOC102724652
Protein Name
P02489
Gene Name URL
CRYAA, LOC102724652
Nucleotide Accession Number
NM_000394.3

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