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MTDH ELISA Kit (Human)

CAT: 0247-OKEH04866-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04866-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Metadherin
Gene Aliases
3D3;3D3/LYRIC; AEG1; AEG-1; astrocyte elevated gene 1; astrocyte elevated gene-1 protein; LYRIC; LYRIC/3D3; lysine-rich CEACAM1 co-isolated protein; Metadherin; metastasis adhesion protein; protein LYRIC.
Gene ID
92140
Accession Number
NP_848927.2
Reactivity
Homo sapiens, Human
Target
Protein LYRIC
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology MTDH ELISA Kit (Human) (OKEH04866) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MTDH has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MTDH is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MTDH captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
1.56-100nmol/L
Sensitivity
0.092nmol/L
Reconstitution
Store as indicated in product manual.
Molecular Weight
64 kDa
Specificity
Natural and recombinant Human Protein LYRIC
Protein Length
582
NCBI Gene Symbol
MTDH
Protein Name
Q86UE4
Gene Name URL
MTDH
Nucleotide Accession Number
NM_178812.3