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Ptpn1 ELISA Kit (Rat)

CAT: 0247-OKEH04845-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04845-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Protein tyrosine phosphatase, non-receptor type 1
Gene Aliases
Protein-tyrosine phosphatase 1B; Ptp; PTP-1B; tyrosine-protein phosphatase non-receptor type 1.
Gene ID
24697
Accession Number
NP_036769.1
Reactivity
Rat, Rattus norvegicus
Target
Tyrosine-protein phosphatase non-receptor type 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Ptpn1 ELISA Kit (Rat) (OKEH04845) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ptpn1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ptpn1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ptpn1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
0.625-40ng/mL
Sensitivity
0.312 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
50 kDa
Specificity
Natural and recombinant Rat Tyrosine-protein phosphatase non-receptor type 1
Protein Length
432
NCBI Gene Symbol
Ptpn1
Protein Name
P20417
Gene Name URL
Ptpn1
Nucleotide Accession Number
NM_012637.2

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