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ADCY10 ELISA Kit (Human)

CAT: 0247-OKEH04831-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04831-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Adenylate cyclase 10
Gene Aliases
3',5'-cyclic AMP synthetase; adenylate cyclase 10, soluble; Adenylate cyclase homolog; adenylate cyclase type 10; AH-related protein; ATP pyrophosphate-lyase; epididymis secretory sperm binding protein Li 7a; germ cell soluble adenylyl cyclase; HCA2; HEL-S-7a; hsAC; SAC; SACI; Sacy; Testicular soluble adenylyl cyclase; testicular soluble adenylyl cyclase (SAC) .
Gene ID
55811
Accession Number
NP_001161221.1
Reactivity
Homo sapiens, Human
Target
Adenylate cyclase type 10
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology ADCY10 ELISA Kit (Human) (OKEH04831) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ADCY10 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ADCY10 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ADCY10 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
78-5000pg/mL
Sensitivity
32 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
187 kDa
Specificity
Natural and recombinant Human Adenylate cyclase type 10
Protein Length
1610
NCBI Gene Symbol
ADCY10
Protein Name
Q96PN6
Gene Name URL
ADCY10
Nucleotide Accession Number
NM_001167749.2

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