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Bax ELISA Kit (Mouse)

CAT: 0247-OKEH04827-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04827-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
BCL2-associated X protein
Gene Aliases
Apoptosis regulator BAX.
Gene ID
12028
Accession Number
NP_031553.1
Reactivity
Mouse, Mus musculus
Target
Apoptosis regulator BAX
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Bax ELISA Kit (Mouse) (OKEH04827) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Bax has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Bax is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Bax captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
31.2-2000pg/mL
Sensitivity
15.6 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
21 kDa
Specificity
Natural and recombinant Mouse Apoptosis regulator BAX
Protein Length
192
NCBI Gene Symbol
Bax
Protein Name
Q07813
Gene Name URL
Bax
Nucleotide Accession Number
NM_007527.3

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