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Hist4h4 ELISA Kit (Mouse)

CAT: 0247-OKEH04805-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04805-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Histone cluster 1, H4h
Gene Aliases
1700024H08Rik; B130044J01Rik; germinal histone H4; Gm11275; H2c8; H4; H4 histone family, member A; H4-16; H4c1; H4c11; H4c12; H4c14; H4c2; H4c3; H4c4; H4c6; H4c8; H4c9; H4f16; Hist1; Hist1h; Hist1h4a; Hist1h4b; Hist1h4c; Hist1h4d; Hist1h4f; Hist1h4h; Hist1h4i; Hist1h4j; Hist1h4k; Hist1h4m; Hist1h4n; Hist2; Hist2h4; Hist4; Hist4h4; histone 1, H4a; histone 1, H4b; histone 1, H4c; histone 1, H4d; histone 1, H4f; histone 1, H4h; histone 1, H4i; histone 1, H4j; histone 1, H4k; histone 2, H4; histone 4, H4; histone cluster 1, H4a; histone cluster 1, H4b; histone cluster 1, H4c; histone cluster 1, H4d; histone cluster 1, H4f; histone cluster 1, H4h; histone cluster 1, H4i; histone cluster 1, H4j; histone cluster 1, H4k; histone cluster 1, H4m; histone cluster 1, H4n; histone cluster 2, H4; histone cluster 4, H4; histone H4; X04652.
Gene ID
100041230, 319155, 319156, 319157, 319158, 319159, 319160, 319161, 320332, 326619, 326620, 69386, 97122
Accession Number
NP_001182350.1
Reactivity
Mouse, Mus musculus
Target
Histone H4
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Hist4h4 ELISA Kit (Mouse) (OKEH04805) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hist4h4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Hist4h4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hist4h4 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
0.156-10ng/mL
Sensitivity
0.093 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
11 kDa
Specificity
Natural and recombinant Mouse Histone H4
Protein Length
103
NCBI Gene Symbol
H4c1, H4c11, H4c12, H4c14, H4c17, H4c18, H4c2, H4c3, H4c4, H4c6, H4c8, H4c9, H4f16
Protein Name
P62806
Gene Name URL
H4c1, H4c11, H4c12, H4c14, H4c17, H4c18, H4c2, H4c3, H4c4, H4c6, H4c8, H4c9, H4f16
Nucleotide Accession Number
NM_001195421.1

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