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Cdk5r1 ELISA Kit (Rat)

CAT: 0247-OKEH04799-96WSize: 96 TestsDry Ice: NoHazardous: No
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CAT#:0247-OKEH04799-96WSize:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Cyclin-dependent kinase 5 regulatory subunit 1
Gene Aliases
CDK5 activator 1; Cdk5r; cyclin-dependent kinase 5 activator 1; Cyclin-dependent kinase 5 regulatory subunit 1; p23; p35; tau protein kinase II 23 kDa subunit; TPKII regulatory subunit.
Gene ID
116671
Accession Number
NP_446343.1
Reactivity
Rat, Rattus norvegicus
Target
Cyclin-dependent kinase 5 activator 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Cdk5r1 ELISA Kit (Rat) (OKEH04799) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cdk5r1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Cdk5r1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cdk5r1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
0.156-10ng/ml
Sensitivity
0.093 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
34 kDa
Specificity
Natural and recombinant Rat Cyclin-dependent kinase 5 activator 1
Protein Length
307
NCBI Gene Symbol
Cdk5r1
Protein Name
P61810
Gene Name URL
Cdk5r1
Nucleotide Accession Number
NM_053891.1

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