AIM2 ELISA Kit (Human)

CAT: 0247-OKEH04784-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH04784-96WSize:96 Well
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CAS Number
7732-18-5
Gene Name
Absent in melanoma 2
Gene Aliases
Absent in melanoma 2; interferon-inducible protein AIM2; PYHIN4.
Gene ID
9447
Accession Number
NP_004824.1
Reactivity
Homo sapiens, Human
Target
Interferon-inducible protein AIM2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology AIM2 ELISA Kit (Human) (OKEH04784) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AIM2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AIM2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample AIM2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
0.312-20ng/mL
Sensitivity
0.15 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
39 kDa
Specificity
Natural and recombinant Human Interferon-inducible protein AIM2
Protein Length
343
NCBI Gene Symbol
AIM2
Protein Name
O14862
Gene Name URL
AIM2
Nucleotide Accession Number
NM_004833.1

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