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Cspg4 ELISA Kit (Rat)

CAT: 0247-OKEH04783-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04783-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Chondroitin sulfate proteoglycan 4
Gene Aliases
Chondroitin sulfate proteoglycan 4; chondroitin sulfate proteoglycan NG2; HSN tumor-specific antigen; membrane-spanning proteoglycan NG2; Ng2.
Gene ID
81651
Accession Number
NP_112284.1
Reactivity
Rat, Rattus norvegicus
Target
Chondroitin sulfate proteoglycan 4
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Cspg4 ELISA Kit (Rat) (OKEH04783) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cspg4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Cspg4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cspg4 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
0.312-20ng/ml
Sensitivity
0.156 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
252 kDa
Specificity
Natural and recombinant Rat Chondroitin sulfate proteoglycan 4
Protein Length
2326
NCBI Gene Symbol
Cspg4
Protein Name
Q00657
Gene Name URL
Cspg4
Nucleotide Accession Number
NM_031022.1