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LCAT ELISA Kit (Human)

CAT: 0247-OKEH04779-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04779-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Lecithin-cholesterol acyltransferase
Gene Aliases
1-alkyl-2-acetylglycerophosphocholine esterase; Lecithin-cholesterol acyltransferase; PAF acetylhydrolase; phosphatidylcholine-sterol acyltransferase; phosphatidylcholine--sterol O-acyltransferase; phospholipid-cholesterol acyltransferase; platelet-activating factor acetylhydrolase; testicular secretory protein Li 24.
Gene ID
3931
Accession Number
NP_000220.1
Reactivity
Homo sapiens, Human
Target
Phosphatidylcholine-sterol acyltransferase
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology LCAT ELISA Kit (Human) (OKEH04779) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LCAT has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LCAT is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LCAT captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
0.156-10ng/mL
Sensitivity
0.098 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
50 kDa
Specificity
Natural and recombinant Human Phosphatidylcholine-sterol acyltransferase
Protein Length
440
NCBI Gene Symbol
LCAT
Protein Name
P04180
Gene Name URL
LCAT
Nucleotide Accession Number
NM_000229.1

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