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MMP14 ELISA Kit (Human)

CAT: 0247-OKEH04765-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04765-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Matrix metallopeptidase 14
Gene Aliases
Matrix metallopeptidase 14 (membrane-inserted) ; matrix metalloproteinase-14; membrane type 1 metalloprotease; Membrane-type matrix metalloproteinase 1; membrane-type-1 matrix metalloproteinase; MMP-14; MMP-X1; MT1MMP; MT1-MMP; MT-MMP; MT-MMP 1; MTMMP1; WNCHRS.
Gene ID
4323
Accession Number
NP_004986.1
Reactivity
Homo sapiens, Human
Target
Matrix metalloproteinase-14
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology MMP14 ELISA Kit (Human) (OKEH04765) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MMP14 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MMP14 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MMP14 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
31.2-2000pg/mL
Recovery
Mean recovery when spiking into Serum and Plasma = 101%
Sensitivity
10 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
66 kDa
Specificity
Natural and recombinant Human Matrix metalloproteinase-14
Protein Length
582
NCBI Gene Symbol
MMP14
Protein Name
P50281
Gene Name URL
MMP14
Nucleotide Accession Number
NM_004995.3

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