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IKBKB ELISA Kit (Human)

CAT: 0247-OKEH04742-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04742-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Inhibitor of nuclear factor kappa B kinase subunit beta
Gene Aliases
I-kappa-B kinase 2; I-kappa-B-kinase beta; IKK2; IKKB; IKK-beta; IMD15; IMD15A; IMD15B; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta; inhibitor of nuclear factor kappa-B kinase subunit beta; NFKBIKB; nuclear factor NF-kappa-B inhibitor kinase beta; serine/threonine protein kinase IKBKB.
Gene ID
3551
Accession Number
NP_001177649.1
Reactivity
Homo sapiens, Human
Target
Inhibitor of nuclear factor kappa-B kinase subunit beta
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology IKBKB ELISA Kit (Human) (OKEH04742) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IKBKB has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IKBKB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IKBKB captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
1.56-100ng/mL
Sensitivity
0.79 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
87 kDa
Specificity
Natural and recombinant Human Inhibitor of nuclear factor kappa-B kinase subunit beta
Protein Length
756
NCBI Gene Symbol
IKBKB
Protein Name
O14920
Gene Name URL
IKBKB
Nucleotide Accession Number
NM_001190720.2

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