CD209 ELISA Kit (Human)

CAT: 0247-OKEH04724-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH04724-96WSize:96 Well
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CAS Number
7732-18-5
Gene Name
CD209 molecule
Gene Aliases
CD209 antigen; CDSIGN; CLEC4L; C-type lectin domain family 4 member L; DC-SIGN; DC-SIGN1; dendritic cell-specific ICAM-3-grabbing non-integrin 1; dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin; dendritic cell-specific intracellular adhesion molecules (ICAM) -3 grabbing non-integrin; hDC-SIGN; HIV gpl20-binding protein.
Gene ID
30835
Accession Number
NP_001138365.1
Reactivity
Homo sapiens, Human
Target
CD209 antigen
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CD209 ELISA Kit (Human) (OKEH04724) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CD209 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CD209 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CD209 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Detection Range
78-5000pg/ml
Sensitivity
40.9 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
46 kDa
Specificity
Natural and recombinant Human CD209 antigen
Protein Length
404
NCBI Gene Symbol
CD209
Protein Name
Q9NNX6
Gene Name URL
CD209
Nucleotide Accession Number
NM_001144893.1

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