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PVRL1 ELISA Kit (Human)

CAT: 0247-OKEH04689-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04689-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Nectin cell adhesion molecule 1
Gene Aliases
CD111; CLPED1; ectodermal dysplasia 4 (Margarita Island type) ; ED4; herpes simplex virus type 1 sensitivity; herpes virus entry mediator C; herpesvirus Ig-like receptor; HIgR; HV1S; HVEC; nectin 1; Nectin cell adhesion molecule 1; nectin-1; OFC7; poliovirus receptor-like 1; poliovirus receptor-related 1 (herpesvirus entry mediator C) ; poliovirus receptor-related protein 1; PRR; PRR1; PVRL1; PVRR; PVRR1; SK-12.
Gene ID
5818
Accession Number
NP_002846.3
Reactivity
Homo sapiens, Human
Target
Nectin-1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology PVRL1 ELISA Kit (Human) (OKEH04689) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PVRL1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for PVRL1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PVRL1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Sensitivity
0.078 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
57 KDa
Specificity
Natural and recombinant Human Poliovirus receptor-related protein 1
Protein Length
517
NCBI Gene Symbol
NECTIN1
Protein Name
Q15223
Gene Name URL
NECTIN1
Nucleotide Accession Number
NM_002855.4

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