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ALDOA ELISA Kit (Human)

CAT: 0247-OKEH04675-96WSize: 96 WellDry Ice: NoHazardous: No
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CAT#:0247-OKEH04675-96WSize:96 Well
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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CAS Number
7732-18-5
Gene Name
Aldolase, fructose-bisphosphate A
Gene Aliases
ALDA; aldolase A, fructose-bisphosphate; epididymis secretory sperm binding protein Li 87p; fructose-1,6-bisphosphate triosephosphate-lyase; fructose-bisphosphate aldolase A; GSD12; HEL-S-87p; lung cancer antigen NY-LU-1; muscle-type aldolase.
Gene ID
226
Accession Number
NP_000025.1
Reactivity
Homo sapiens, Human
Target
Fructose-bisphosphate aldolase A
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology ALDOA ELISA Kit (Human) (OKEH04675) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ALDOA has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for ALDOA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ALDOA captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma , Urine , tissue homogenates and Cell culture supernates and Other biological fluids
Detection Range
1.56-100U/mL
Sensitivity
0.78U/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
39 KDa
Specificity
Natural and recombinant Human Fructose-bisphosphate aldolase A
Protein Length
364
NCBI Gene Symbol
ALDOA
Protein Name
P04075
Gene Name URL
ALDOA
Nucleotide Accession Number
NM_000034.3

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