Ccl25 ELISA Kit (Mouse)

CAT: 0247-OKEH04673-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH04673-96WSize:96 Well
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CAS Number
7732-18-5
Gene Name
Chemokine (C-C motif) ligand 25
Gene Aliases
A130072A22Rik; AI852536; C-C motif chemokine 25; Chemokine TECK; CKb15; Scya; Scya25; small inducible chemokine 25; small inducible cytokine A25; Small-inducible cytokine A25; TECK; thymus-expressed chemokine.
Gene ID
20300
Accession Number
NP_033164.1
Reactivity
Mouse, Mus musculus
Target
C-C motif chemokine 25
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Ccl25 ELISA Kit (Mouse) (OKEH04673) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ccl25 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Ccl25 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ccl25 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
31.2-2000pg/mL
Sensitivity
15.6 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
17 KDa
Specificity
Natural and recombinant Mouse C-C motif chemokine 25
Protein Length
144
NCBI Gene Symbol
Ccl25
Protein Name
O35903
Gene Name URL
Ccl25
Nucleotide Accession Number
NM_009138.3

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