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Abca1 ELISA Kit (Mouse)

CAT: 0247-OKEH04671-96WSize: 96 WellDry Ice: NoHazardous: No
CAT#:0247-OKEH04671-96WSize:96 Well
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CAS Number
7732-18-5
Gene Name
ATP-binding cassette, sub-family A (ABC1), member 1
Gene Aliases
ABC; Abc1; ABC-1; ATP-binding cassette 1; ATP-binding cassette sub-family A member 1; ATP-binding cassette transporter 1; phospholipid-transporting ATPase ABCA1.
Gene ID
11303
Accession Number
NP_038482.3
Reactivity
Mouse, Mus musculus
Target
ATP-binding cassette sub-family A member 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Abca1 ELISA Kit (Mouse) (OKEH04671) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Abca1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Abca1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Abca1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Sensitivity
0.089 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
254 KDa
Specificity
Natural and recombinant Mouse ATP-binding cassette sub-family A member 1
Protein Length
2261
NCBI Gene Symbol
Abca1
Protein Name
P41233
Gene Name URL
Abca1
Nucleotide Accession Number
NM_013454.3

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